Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

14.0K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.0K
Membrane Fluidity01:23

Membrane Fluidity

170.6K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
170.6K
Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model01:09

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

651
Various dissolution theories provide insight into the factors that influence the dissolution rate. Danckwerts' Model suggests that turbulence, rather than a stagnant layer, characterizes the dissolution medium at the solid-liquid interface. In this model, the agitated solvent contains macroscopic packets that move to the interface via eddy currents, facilitating the absorption and delivery of the drug to the bulk solution. The regular replenishment of solvent packets maintains the...
651
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

9.3K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
9.3K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.3K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.

ACS applied materials & interfaces·2026
Same author

Chain Length as a Molecular Determinant of Hydrogen-Bond Dynamics in Biocondensates.

The journal of physical chemistry letters·2026
Same author

Solvent Reorganization in Stabilized Protein-Polymer Conjugates Visualized by Two-Dimensional Infrared and Nuclear Magnetic Resonance Spectroscopy.

JACS Au·2026
Same author

Low-cost calculation and analysis of 2D IR spectra of model diiron trinitrosyl complexes in the NO stretch region with vibrational perturbation theory.

Physical chemistry chemical physics : PCCP·2026
Same author

Membrane Composition Reshapes the Folding Landscape of a pH-Responsive Peptide.

The journal of physical chemistry letters·2025
Same author

Machine learning potentials accurately reproduce vibrational dynamics in complex environments.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Dec 10, 2025

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.9K

Ultrafast Dynamics at Lipid-Water Interfaces.

Jennifer C Flanagan1, Mason L Valentine1, Carlos R Baiz1

  • 1Department of Chemistry, University of Texas at Austin, 105 East 24th Street Stop A5300, Austin, Texas 78712-1224, United States.

Accounts of Chemical Research
|September 1, 2020
PubMed
Summary

Ultrafast spectroscopy reveals that lipid membrane interfaces have slower water dynamics than bulk water due to disrupted hydrogen bonds. These dynamics are influenced by headgroup structure and interactions with peptides or ions.

More Related Videos

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.4K
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
07:54

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

8.3K

Related Experiment Videos

Last Updated: Dec 10, 2025

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.9K
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.4K
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
07:54

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

8.3K

Area of Science:

  • Biophysical Chemistry
  • Interface Science
  • Spectroscopy

Background:

  • Lipid membranes are crucial cellular compartments, regulating biological functions through protein dynamics.
  • Understanding lipid-water interface dynamics is key to molecular complexities, from phase separation to hydrogen bond networks.

Purpose of the Study:

  • To review novel applications of ultrafast spectroscopy in studying lipid membranes.
  • To highlight recent experimental findings on interfacial dynamics and molecular interactions.

Main Methods:

  • Utilizing ultrafast vibrational spectroscopies like two-dimensional infrared (2D IR) and vibrational sum-frequency generation (VSFG).
  • 2D IR spectroscopy offers bond-centered dynamics with subpicosecond resolution.
  • VSFG spectroscopy probes water modes and headgroup ordering at the interface.

Main Results:

  • Water dynamics at the lipid-water interface are slower than bulk water due to disrupted hydrogen bonds.
  • Interfacial dynamics are perturbed by peptides, ions, osmolytes, and cosolvents.
  • Headgroup structure and hydrophobic acyl chain interactions dictate interfacial environments.

Conclusions:

  • Ultrafast spectroscopy provides essential molecular insights into lipid-water interface biophysics.
  • Challenges remain in spectral interpretation and modeling complex membrane environments.
  • Combining spectroscopy with simulations enhances understanding of multicomponent membranes.