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Updated: Dec 10, 2025
Author Spotlight: Utilizing Infraorbital Nerve Ligation in Mice for Investigating Trigeminal Neuropathic Pain and Treatment Strategies
Published on: March 8, 2024
Sensory, Affective, and Cognitive Effects of Trigeminal Injury in Mice
Claudia Daniela Montes Angeles1, Rey David Andrade Gonzalez1, Elias Perrusquia Hernandez1
1Student/Resident, Sección de neurobiología de las sensaciones orales, Laboratorio de Investigación odontológica, Clínica Universitaria de Salud Integral Almaraz, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, México.
Purpose:
To characterize adequate study of chronic neuropathic orofacial pain induced by a mental nerve injury in a mouse model, we propose a behavioral assessment of its dimensions: sensory, affective, and cognitive.
Materials And Methods:
Trigeminal injury was induced by a chronic mental nerve constriction (MnC). Behavioral tests were conducted to assess the different dimensions of pain and to evaluate the general well-being of mice.
Results:
Rodents who went through MnC showed signs of mechanical hyperalgesia and increased escape/avoidance behavior. They showed no alterations in general well-being behaviors, yet the injury was sufficient to induce impairment in the ability to adapt to the environmental requirements.
Conclusions:
MnC injury is an efficient model for the study of orofacial pain in mice, capable of inducing impairment in the different dimensions of pain. Intensity and temporality of its effects make our model less aggressive, yet effective to generate cognitive impairment. This work provides a solid foundation for the study of the neural circuits involved in the processing of neuropathic orofacial pain.
