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Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
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Overview of Nitrogen Metabolism01:20

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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
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Key Elements for Plant Nutrition02:35

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Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
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The Nitrogen Cycle01:49

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Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
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Overview of Metabolism01:40

Overview of Metabolism

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Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
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The Roles of Bacteria and Fungi in Plant Nutrition02:11

The Roles of Bacteria and Fungi in Plant Nutrition

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Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
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Related Experiment Video

Updated: Dec 10, 2025

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
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Improving nitrogen use efficiency by manipulating nitrate remobilization in plants.

Kuo-En Chen1,2, Hui-Yu Chen2, Ching-Shan Tseng3

  • 1Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.

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|September 2, 2020
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Enhancing nitrate remobilization in plants boosts nitrogen use efficiency (NUE). A new strategy using a modified nitrate transporter improved plant growth and yield, offering a sustainable approach to crop production.

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Area of Science:

  • Plant Biology
  • Agricultural Science
  • Biotechnology

Background:

  • Nitrogen use efficiency (NUE) is crucial for sustainable agriculture, impacting crop yield and environmental health.
  • Nitrate remobilization, particularly via the NRT1.7 transporter, is vital for plant growth under nitrogen limitation.
  • The nrt1.7 mutant shows impaired growth, highlighting the importance of NRT1.7-mediated nitrate allocation.

Purpose of the Study:

  • To enhance nitrogen use efficiency (NUE) by improving nitrate remobilization.
  • To investigate the potential of a hyperactive chimeric nitrate transporter (NC4N) to boost nitrate recycling.
  • To assess the impact of enhanced nitrate remobilization on plant growth and yield in various crop species.

Main Methods:

  • Genetic engineering of Arabidopsis, tobacco, and rice by introducing the NRT1.7p::NC4N::3' construct into the nrt1.7 mutant background.
  • Utilizing 15NO3- tracing to quantify nitrate remobilization from source to sink tissues.
  • Measuring nitrate accumulation in different plant organs and evaluating overall plant growth and yield parameters.

Main Results:

  • Transgenic plants (NRT1.7p::NC4N::3') exhibited increased nitrate accumulation in younger leaves.
  • 15N tracing confirmed enhanced remobilization of nitrate into sink tissues in transgenic lines.
  • Arabidopsis, tobacco, and rice plants engineered with the construct demonstrated improved growth and/or yield.

Conclusions:

  • Enhancing source-to-sink nitrate remobilization is a viable strategy for improving nitrogen use efficiency (NUE).
  • The hyperactive chimeric transporter NC4N, under the NRT1.7 promoter, effectively boosts nitrate recycling.
  • This approach offers a promising avenue for increasing crop production sustainably.