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Published on: July 19, 2018
Chemokine receptors on peripheral blood T lymphocytes in children on peritoneal dialysis
Maria Szczepańska1, Łukasz Sędek2, Joanna Bulsa3
1Department of Pediatrics, 49613Faculty of Medical Sciences in Zabrze, SUM in Katowice, Poland.
Insights
This study investigated T-cell chemokine receptor expression in children with chronic kidney disease (CKD) undergoing peritoneal dialysis (PD). Results showed altered T-cell numbers and receptor expression, suggesting potential impacts on immune function in pediatric CKD patients on PD.
Area of Science:
- Immunology
- Nephrology
- Pediatrics
Background:
- Chronic kidney disease (CKD) is associated with immune cell dysfunction.
- T-cells play a critical role in immune responses, including lymphocytic migration and infiltration.
- Limited data exists on chemokine receptor expression on T-cells in pediatric CKD patients, especially those on peritoneal dialysis (PD).
Purpose of the Study:
- To analyze T-cell subsets and their surface chemokine receptor expression in children with CKD treated with PD.
- To compare chemokine receptor expression patterns between children on PD and healthy controls.
Main Methods:
- Multiparameter flow cytometry was used to analyze T-cell subsets.
- Absolute numbers and percentages of T-cells expressing specific chemokine receptors (CCR4, CCR5, CCR7, CXCR3, CXCR4) and their combinations were quantified.
- The study included 47 children undergoing PD.
Main Results:
- Children on PD exhibited lower absolute numbers of total T-cells and specific subsets expressing CCR5, CXCR4, and CCR4.
- Higher percentages of T-cells expressing CD4, CCR7, and CXCR3 were observed in the PD group.
- Mean fluorescent intensity for several chemokine receptors was lower in children on PD compared to healthy controls.
- Correlations were found between T-cell subpopulations, weekly creatinine clearance, and PD treatment efficacy (KT/Vurea).
Conclusions:
- The study did not confirm earlier T-cell senescence in children with CKD on PD, warranting further investigation.
- An increased percentage of T-cells expressing CCR7 may be linked to enhanced proliferation activity in these children.
- Findings highlight alterations in T-cell chemokine receptor expression in pediatric CKD patients on PD, suggesting potential immune dysregulation.
Background:
Immune cell dysfunction is listed among complications resulting from chronic kidney disease (CKD). It could be associated with T-cells, which play a role in the lymphocytic migration and infiltration. However, the data on chemokine receptors expression on T-cells in patients with CKD particularly treated with peritoneal dialysis (PD) are still limited.
Methods:
The study aimed at multiparameter flow-cytometric analysis of the absolute numbers and percentage of T-cell subsets with surface chemokine receptors (CCR4, CCR5, CCR7, CXCR3, and CXCR4) or receptors' combinations in 47 children treated with PD.
Results:
We found lower absolute numbers of total T lymphocytes, lymphocytes with surface CCR5, CXCR4+CCR5, CXCR3+CCR5 antigens and T-cells with CCR4, CCR4+CD4, CXCR3, CXCR3+CD4, and CD8 receptors. Lymphocytes T with CD4, CCR7, CD28+CCR7, CXCR3+CD8 antigens showed higher percentage in children on PD as compared to healthy children and opposite percentage values of CCR4+, CCR4+CD4+, CXCR3+ T lymphocytes were diminished. Mean fluorescent intensity for CCR7+, CCR7+CD45RO+, CCR7+CD28+, CXCR4+CD4+, CCR5+CD4+, CCR4+, CCR4+CD4+ T-cells was lower in the PD group than in healthy children. The analysis of correlation between T lymphocyte subpopulations with chemokine receptors and other parameters revealed positive correlation of CCR7+ and CCR7+CD28+ T-cells and weekly creatinine clearance, negative correlation between the percentage of CD45RO+CCR7 antigen positive T-cells and KT/Vurea.
Summary:
In conclusion, we could not confirm the phenomenon of earlier senescence of T-cells in children with CKD on PD treatment. This still requires further investigation. The higher percentage of T-cells with CCR7 surface receptor could be responsible for the increase of proliferation activity in this group of children.
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