A fluorescence sensing method for brilliant blue with gold nanoclusters based on the inner filter effect

Area of Science:

• Analytical Chemistry
• Nanotechnology
• Food Science

Background:

• The inner filter effect (IFE) is a phenomenon where one substance absorbs light emitted by another.
• Gold nanoclusters (AuNCs) exhibit unique fluorescence properties.
• Accurate detection of food dyes like Brilliant Blue (BB) is crucial for quality control.

Purpose of the Study:

• To develop a simple, rapid, and sensitive method for detecting Brilliant Blue (BB).
• To utilize the inner filter effect (IFE) between BB and AuNCs for quantitative analysis.
• To validate the method's applicability in real food samples.

Main Methods:

• Utilizing the fluorescence quenching of AuNCs induced by BB due to IFE.
• Optimizing reaction conditions for BB detection.
• Quantifying BB using fluorescence spectroscopy.
• Analyzing BB content in various food matrices (hard candy, chocolate, dried blueberries).

Main Results:

• A linear detection range for BB was established from 0.05 to 7.5 μM.
• A low detection limit of 0.0167 μM was achieved.
• The assay demonstrated successful application in detecting BB in diverse food samples.

Conclusions:

• The developed fluorescence quenching method based on IFE is effective for BB detection.
• The assay shows high sensitivity and applicability to real-world food samples.
• This method holds potential for future food quality control applications.