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MicroRNA-183 as a Novel Regulator Protects Against Cardiomyocytes Hypertrophy via Targeting TIAM1

Fu-Han Gong1, Xi-Lu Chen2, Quan Zhang3

  • 1Department of Cardiology, Tongren Municipal People's Hospital, Tongren, China.

American Journal of Hypertension
|September 2, 2020
PubMed
Abstract

Insights

MicroRNA-183 (miR-183) is downregulated in cardiac hypertrophy. Restoring miR-183 levels protects against heart cell enlargement by targeting TIAM1, offering a potential therapeutic strategy.

Area of Science:

  • Cardiovascular Biology
  • Molecular Cardiology
  • Epigenetics

Background:

  • MicroRNAs regulate cardiac hypertrophy pathogenesis.
  • miR-183, known as a tumor suppressor, is downregulated in cardiac hypertrophy.

Purpose of the Study:

  • To investigate the role of miR-183 in cardiomyocyte hypertrophy.
  • To identify the molecular targets of miR-183 in the context of cardiac hypertrophy.

Main Methods:

  • Cardiac hypertrophy model induced by Angiotensin II (Ang II) in vitro.
  • Transfection of neonatal rat ventricular cardiomyocytes with miR-183 mimic.
  • Bioinformatics analysis and luciferase reporter assays to identify miR-183 targets.

Main Results:

  • miR-183 expression was significantly decreased in hypertrophic cardiomyocytes.
  • Overexpression of miR-183 attenuated cardiomyocyte size and prohypertrophic gene expression.
  • TIAM1 was identified as a direct target of miR-183, with its expression reduced by miR-183 mimic transfection.

Conclusions:

  • miR-183 acts as a cardioprotective regulator in cardiac hypertrophy.
  • The protective effect of miR-183 is mediated through direct regulation of TIAM1.
Keywords:
TIAM1angiotensin IIblood pressurecardiomyocyteshypertensionmiR-183

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