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Updated: Dec 10, 2025

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Synthesis and Characterization of Cobalt(II) and Manganese(II) Schiff Base Complexes: Metal Effect on the Binding
Nafiseh Jahandari1, Fatemeh S Mohseni-Shahri2, Farid Moeinpour1
1Department of Chemistry, Islamic Azad University, Bandar Abbas Branch, Bandar Abbas, Iran.
Abstract:
In this study, two metal Schiff base complexes (cobalt(II) and manganese(II), were synthesized and characterized by 1H-NMR and FT-IR analyses. The in vitro binding behavior of this complexes with β-Casein (β-CN) was investigated by using biophysical techniques. For evaluation the thermodynamics parameters of interaction between Schiff base complexes and β-CN,the fluorescence data at different temperatures were done. The results showed that the intrinsic fluorescence of the β-CN was quenched by increasing the complexes through the dynamic quenching mechanism. Also, these complexes demonstrated a considrable binding affinity to β-CN and the process is mainly entropy driven. Fluorescence resonance energy transfer was used in order to estimating the distance between donor (β-CN) and acceptor (complex). Results demonstrated that the sequence of tendency of the complexes to β-CN was as follows: Mn-Schiff base complex > Co-Schiff base complex. All these experimental results propose that β-CN might act as carrier protein for Co(II) and Mn (II) complexes to deliver it to the target molecules.
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