Adaptive high-dynamic-range Fourier ptychography microscopy data acquisition with a red-green-blue camera

Area of Science:

• Microscopy
• Optical Imaging
• Computational Imaging

Background:

• Fourier ptychography microscopy (FPM) enables wide-field, high-resolution imaging by combining low-resolution (LR) images in the Fourier domain.
• FPM typically requires capturing numerous images with varying illumination angles or positions.
• Simultaneous phase recovery is a key advantage of FPM.

Purpose of the Study:

• To develop an adaptive high-dynamic-range (HDR) method for low-resolution (LR) image acquisition in Fourier ptychography microscopy.
• To achieve HDR imaging using a standard red, green, and blue (RGB) camera with single exposure per illumination.
• To enhance the efficiency and applicability of FPM for challenging samples.

Main Methods:

• Utilizing the spectral characteristics of an RGB camera's color channels for HDR image reconstruction.
• Illuminating the sample with monochromatic light from Light-Emitting Diodes (LEDs).
• Combining raw data from the red, green, and blue channels to reconstruct an HDR image for each LED illumination.

Main Results:

• Demonstrated successful acquisition of adaptive high-dynamic-range (HDR) low-resolution (LR) images.
• Validated the method's feasibility and performance through experimental results.
• Achieved HDR imaging with single exposure per LED illumination, simplifying the acquisition process.

Conclusions:

• The proposed method effectively enables adaptive HDR LR image acquisition for Fourier ptychography microscopy.
• This approach enhances FPM by allowing single-exposure HDR imaging, improving efficiency.
• The technique shows promise for imaging samples with significant intensity variations.