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Related Concept Videos

MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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Computational Methods and Software Tools for Functional Analysis of miRNA Data.

Adrian Garcia-Moreno1, Pedro Carmona-Saez1,2

  • 1Bioinformatics Unit, Centre for Genomics and Oncological Research (GENyO)-Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, 18016 Granada, Spain.

Biomolecules
|September 3, 2020
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) regulate gene expression and biological processes. This review details two miRNA enrichment analysis workflows, discussing tools and databases for metazoan miRNA research.

Keywords:
databasesenrichmentfunctional analysismiRNAncRNAtools

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression involved in numerous biological processes.
  • High-throughput sequencing enables large-scale discovery and characterization of miRNAs.
  • Enrichment analysis is vital for understanding miRNA involvement in biological pathways.

Purpose of the Study:

  • To review and compare two primary workflows for miRNA enrichment analysis.
  • To highlight statistical methods, software, databases, and annotation resources for metazoan miRNA studies.
  • To address limitations of traditional target-based enrichment analysis.

Main Methods:

  • Description of target gene-based enrichment analysis.
  • Description of miRNA annotation-based enrichment analysis.
  • Discussion of statistical tests, software, and databases for both approaches.

Main Results:

  • Comparison of the strengths and limitations of target gene vs. miRNA annotation workflows.
  • Identification of key resources for performing miRNA enrichment analysis.
  • Overview of current trends and alternative methods in miRNA functional analysis.

Conclusions:

  • MiRNA enrichment analysis is essential for functional genomics.
  • Both target gene and miRNA annotation workflows have utility, with alternatives emerging.
  • Accurate functional annotation and appropriate tool selection are critical for metazoan miRNA research.