3D micro fractal pipettes for capillary based robotic liquid handling

Area of Science:

• Materials Science
• Biotechnology
• Microfluidics

Background:

• Miniaturized and mobile liquid handling devices are crucial for advancing biological and clinical applications.
• Conventional methods like manual or automated pipetting face limitations in precision and portability.
• There is a need for innovative solutions to enhance liquid handling efficiency and versatility.

Purpose of the Study:

• To introduce and evaluate the micro fractal pipette as a novel device for miniaturized liquid handling.
• To demonstrate the efficient liquid transfer capabilities of the micro fractal pipette.
• To highlight its potential for revolutionizing biological and clinical applications.

Main Methods:

• Fabrication of the micro fractal pipette using epoxy polymer via 3D nanoprinting (two-photon absorption polymerization).
• Achieving sub-micrometer resolution in the printing process.
• Demonstration of liquid handling performance between a source droplet and a hydrogel substrate.

Main Results:

• The micro fractal pipette exhibits high porosity (78%) and an 8.5 times larger cavity surface area compared to a full pyramid.
• Biomimetic inner cavity microchannel networks facilitate a low pressure drop, enhancing liquid handling efficiency.
• Successful demonstration of efficient liquid transfer to a hydrogel substrate.

Conclusions:

• The micro fractal pipette is a promising candidate for innovative, miniaturized, and mobile liquid handling systems.
• Its unique fractal design and fabrication method contribute to superior performance.
• The device holds significant potential for diverse applications in biology and clinical settings.