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Related Experiment Video

Updated: Dec 10, 2025

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus
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Standard Immunization of Rabbits.

Edward A Greenfield

    Cold Spring Harbor Protocols
    |September 3, 2020
    PubMed
    Summary

    This study details optimal antigen dosages and injection schedules for immunizing rabbits to produce high-titer antiserum. Rabbits require specific antigen amounts and adjuvant use for effective antibody production and serum collection.

    Area of Science:

    • Immunology
    • Animal Models

    Background:

    • Antibody production in rabbits is crucial for various research applications.
    • Standardized protocols are needed for efficient and reproducible antiserum generation.

    Purpose of the Study:

    • To define optimal antigen doses and immunization schedules for rabbits.
    • To provide guidelines for maximizing serum antibody titers and collection yields.

    Main Methods:

    • Biweekly injections of purified antigen, cultured cells, or cDNA.
    • Use of adjuvants (Complete Freund's Adjuvant initially, then Incomplete Freund's Adjuvant or saline).
    • Regular booster injections and serum collection at specific intervals post-boost.

    Main Results:

    • Recommended antigen doses range from 10 μg to 1 mg per injection, depending on antigen type.

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  • Adjuvants are used for initial immunizations; subsequent boosts use saline or Incomplete Freund's Adjuvant.
  • Booster injections every 6 weeks yield 20-40 mL of serum per rabbit bleed, approximately 10-12 days post-boost.

    • Conclusions:

    • A defined immunization strategy ensures effective antibody production in rabbits.
    • Consistent protocols for antigen administration and serum collection are key for reliable antiserum generation.