Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Replication02:40

DNA Replication

57.0K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
57.0K
The Replisome03:01

The Replisome

37.6K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.6K
Replication in Eukaryotes02:31

Replication in Eukaryotes

196.3K
Overview
196.3K
Replication in Prokaryotes02:35

Replication in Prokaryotes

95.5K
Overview
95.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.5K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.5K
DNA Microarrays02:34

DNA Microarrays

20.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision meets speed through an FPGA-based natively sparse Ising machine for combinatorial optimization.

Nature communications·2026
Same author

A Triple-Signal Output Lateral Flow Platform Leveraging CRISPR/Cas12a and Nanozyme Cascades for Ultra-Sensitive Aflatoxin B1 Detection.

ACS sensors·2026
Same author

Does Testosterone Affect Cognitive Reflection? Evidence From a Double-Blind, Randomized Controlled Study of 1,000 Participants.

Psychological science·2026
Same author

Portable cold-assisted head-space solid-phase microextraction coupled with GC-MS/MS for sensitive determination of trace polychlorinated naphthalenes in water.

Journal of chromatography. A·2026
Same author

A reporting checklist for large language models in behavioural science.

Nature human behaviour·2026
Same author

Scientific workflow in experimental economics.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

Related Experiment Video

Updated: Dec 10, 2025

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
11:19

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Published on: May 4, 2015

11.6K

Are replication rates the same across academic fields? Community forecasts from the DARPA SCORE programme.

Michael Gordon1, Domenico Viganola2, Michael Bishop3

  • 1New Zealand Institute for Advanced Study, Massey University, Auckland, New Zealand.

Royal Society Open Science
|September 3, 2020
PubMed
Summary

Researchers predict increasing replication rates in social sciences over time. Economics is expected to have the highest replication rate, while psychology and education are predicted to have the lowest.

Keywords:
forecastingreplicationscience policy

More Related Videos

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
10:17

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Published on: November 15, 2024

1.5K
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

9.9K

Related Experiment Videos

Last Updated: Dec 10, 2025

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
11:19

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Published on: May 4, 2015

11.6K
Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
10:17

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Published on: November 15, 2024

1.5K
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

9.9K

Area of Science:

  • Social and behavioral sciences
  • Empirical research

Background:

  • The 'Systematizing Confidence in Open Research and Evidence' (SCORE) program by DARPA aims to quantify the reliability of research findings.
  • Assessing the reproducibility of scientific claims is crucial for advancing knowledge.

Purpose of the Study:

  • To generate confidence scores for empirical research claims in social and behavioral sciences.
  • To forecast the likelihood of independent replication for various research findings.

Main Methods:

  • Utilizing prediction markets and surveys to forecast replication outcomes.
  • Gathering expert opinions on the expected replication rates across different academic disciplines.

Main Results:

  • Participants anticipate an increase in replication rates over time.
  • Economics is projected to have the highest replication rate (58%), followed by psychology and education (42%).

Conclusions:

  • The study provides insights into the academic community's perception of the replication crisis.
  • Forecasts highlight expected variations in replication reliability across different scientific fields.