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Updated: Dec 10, 2025

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
Published on: May 1, 2017
Quantification of Fatty Acids and their Regioisomeric Distribution in Triacylglycerols from Porcine and Bovine
Geir Kildahl-Andersen1,2, Eli Gjerlaug-Enger3, Frode Rise1
1Department of Chemistry, University of Oslo, P.O. Box 1033 Blindern, 0315, Oslo, Norway.
Abstract:
The uptake of specific fatty acids in humans is dependent on their position on the glycerol backbone. There is a great interest in methods that can access this information fast and accurately. By way of high-resolution NMR, we have analyzed TAG extracted from pig and beef tissues and obtained quantitative data for the composition and regioisomeric distribution of all major unsaturated fatty acids usually found in these source materials, using a combination of manual integration and deconvolution of 13 C NMR spectra. In addition, we have developed a method for determining composition and regioisomeric distribution of the two main saturated fatty acids found in pork (16:0, 18:0). The results are discussed in relation to species-specific genetic characteristics of fatty acid and TAG biosynthesis. The developed method could support decisions related to breeding for desired fatty acid profiles, and stimulate further methodology developments using high field NMR.
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