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Related Concept Videos

Factors Affecting Protein-Drug Binding: Protein-Related Factors01:20

Factors Affecting Protein-Drug Binding: Protein-Related Factors

575
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
575
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

15.1K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
15.1K
Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

9.0K
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
9.0K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

667
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
667
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K
Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

1.8K
Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
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Related Experiment Video

Updated: Feb 9, 2026

Handling and Assessment of Human Primary Prostate Organoid Culture
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Handling and Assessment of Human Primary Prostate Organoid Culture

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Estramustine-binding protein in rat and human prostate.

B Forsgren1

  • 1Department of Cancer Pharmacology, AB LEO Research Laboratories, Sweden.

Scandinavian Journal of Urology and Nephrology. Supplementum
|January 1, 1988
PubMed
Summary

Estramustine phosphate concentrates in rat prostate tissue. A specific protein binding estramustine was identified in rat and human prostates, suggesting a role in the drug's mechanism of action.

Area of Science:

  • Oncology
  • Pharmacology
  • Biochemistry

Background:

  • Estramustine phosphate is a chemotherapy agent derived from estradiol.
  • Its distribution and mechanism of action in prostate cancer require further elucidation.

Purpose of the Study:

  • To investigate the tissue distribution of estramustine phosphate in rats.
  • To identify and characterize a protein that binds estramustine in the prostate.

Main Methods:

  • Radiolabeling of estramustine phosphate and estradiol with tritium.
  • Tissue concentration measurements in rat prostate.
  • Protein binding studies and characterization of the estramustine-binding protein.

Main Results:

  • Estramustine phosphate showed higher radioactivity concentration in rat prostate compared to estradiol.

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  • A specific estramustine-binding protein was identified in rat prostate.
  • This binding protein was also found in human prostate tissue.

    • Conclusions:

    • Estramustine phosphate exhibits preferential accumulation in the prostate.
    • The presence of an estramustine-binding protein in human prostate suggests a potential role in the drug's therapeutic effect.
    • Further research into this protein may reveal insights into estramustine phosphate's mechanism of action.