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Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages.

Maria Papadaki1,2, Elisavet Kaitetzidou1, Constantinos C Mylonas1

  • 1Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, P.O. Box 2214, 71003, Iraklion, Crete, Greece.

Marine Biotechnology (New York, N.Y.)
|September 3, 2020
PubMed
Summary

Non-coding RNAs (ncRNAs) play a key role in fish reproduction. This study identified distinct ncRNA profiles in mature and immature seabream gonads, revealing their involvement in oocyte development and reproductive performance.

Keywords:
Differential expressionEpigeneticsGonad maturationNon-coding RNATeleost

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Area of Science:

  • * Reproductive biology and molecular endocrinology in aquaculture.
  • * Genomics and transcriptomics of non-coding RNAs in fish.

Background:

  • * Non-coding RNAs (ncRNAs) are crucial regulators in biological pathways, including reproduction.
  • * Understanding ncRNA dynamics in fish gonads is essential for improving aquaculture reproductive success.
  • * Sharpsnout seabream (Diplodus puntazzo) exhibits reproductive dysfunction in captivity, necessitating investigation into underlying molecular mechanisms.

Purpose of the Study:

  • * To investigate the abundance and expression patterns of ncRNAs in mature and immature female sharpsnout seabream gonads.
  • * To identify specific ncRNA biotypes and their differential expression related to gonad maturation.
  • * To predict potential targets of differentially expressed ncRNAs involved in oocyte development.

Main Methods:

  • * RNA sequencing was employed to analyze ncRNA populations in gonads of different maturation stages.
  • * ncRNA biotypes, including microRNAs (miRNAs) and PIWI RNAs (piRNAs), were identified and quantified.
  • * Bioinformatic tools were used to predict target genes and long non-coding RNAs (lncRNAs) for identified miRNAs.

Main Results:

  • * Distinct size distributions for miRNAs and PIWI RNAs were observed in sharpsnout seabream gonads.
  • * Differential expression of miRNAs, PIWI RNAs, and ribosomal RNAs was detected between mature and immature gonads.
  • * Ten miRNAs showed significant differential expression, with 2 higher in immature and 8 in mature gonads, targeting genes involved in oocyte development processes.

Conclusions:

  • * A gonad maturation-biased ncRNA profile exists in sharpsnout seabream.
  • * ncRNAs, particularly miRNAs, play a significant role in regulating ovarian physiology and reproductive performance in fish.
  • * Specific ncRNA biotypes contribute distinctly to oocyte development and maturation.