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A modified assay for cyclosporin in blood using solid-phase extraction with high-performance liquid chromatography
B G Charles1, R L Norris, P J Ravenscroft
1Department of Clinical Pharmacology, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
Therapeutic Drug Monitoring
|January 1, 1988
Summary
A new high-performance liquid chromatography method accurately measures cyclosporin in blood using reusable cartridges and a durable column. This validated assay is suitable for clinical use, detecting levels down to 50 ng/ml.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
Background:
- Accurate measurement of cyclosporin is crucial for therapeutic drug monitoring.
- Existing methods may have limitations in efficiency or cost-effectiveness.
Purpose of the Study:
- To describe and evaluate a modified high-performance liquid chromatography (HPLC) procedure for measuring cyclosporin in whole blood for clinical applications.
Main Methods:
- Utilized solid-phase extraction (SPE) cartridges for sample preparation, noting their reusability.
- Employed a reverse-phase analytical column with a demonstrated lifespan exceeding 1,000 injections at 70°C.
- Validated linearity, imprecision, and minimum measurable concentration.
Main Results:
- Cyclosporin A and D (internal standard) elution times were 5.6 and 7.6 minutes, respectively.
- Calibration plots demonstrated linearity from 50 ng/ml to at least 2,000 ng/ml.
- Within-day and between-day imprecision were below 9% (coefficient of variation), with a minimum measurable concentration of 50 ng/ml.
Conclusions:
- The modified HPLC method is reliable and efficient for clinical cyclosporin measurement.
- The use of reusable SPE cartridges and a long-lasting analytical column enhances the practicality and cost-effectiveness of the assay.