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Updated: Dec 10, 2025

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Identification of A functional region in Bombyx mori nucleopolyhedrovirus VP39 that is essential for nuclear actin
Jianjia Zhang1, Yang Li1, Shudi Zhao1
1College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Abstract:
Nuclear actin polymerization plays an indispensable role in the nuclear assembly of baculovirus nucleocapsid, but the underlying viral infection-mediated mechanism remains unclear. VP39 is the major protein in baculovirus capsid, which builds the skeleton of the capsid tubular structure. VP39 is suggested in previous studies to interact with cellular actin and mediate actin polymerization. However, it is unclear about the role of VP39 in mediating nuclear actin polymerization. Results in this study indicated that vp39 deletion abolished nuclear actin polymerization, which was recovered after vp39 repair, revealing the essential part of VP39 in nuclear actin polymerization. Furthermore, a series of mutants with vp39 deletions were constructed to analyze the important region responsible for nuclear actin polymerization. In addition, intracellular localization analysis demonstrated that the amino acids 192-286 in VP39 C-terminal are responsible for nuclear actin polymerization.
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