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MiR-214 regulates fracture healing through inhibiting Sox4 and its mechanism
Zhaoxu Xin1, Defu Cai2, Jingchun Wang3
1Department of Orthopaedics, The Third Affiliated Hospital of Qiqihar Medical University, P.R. China.
Objective:
To investigate the expression of micro ribonucleic acid (miR)-214 in the bone tissue and blood of patients with fragility fracture.
Methods:
The expression of miR-214 was detected via quantitative reverse transcription-polymerase chain reaction. The effect of miR-214 on proliferation and apoptosis of osteoblasts were detected via methyl thiazolyl tetrazolium assay and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining.
Results:
The expression of miR-214 in the bone tissue and blood of patients with fragility fracture significantly declined. miR-214 could promote the proliferation of osteoblasts and inhibited the apoptosis of osteoblasts. miR-214 is involved in fracture healing through inhibiting Sox4 and promoting phosphorylation of PI3K/AKT pathway. The expression of BSP in cells treated with miR-214 mimics was significantly increased to 2.5-fold (p=0.0168), while the expression of BSP in cells treated with miR-214 AMO was significantly decreased, reduced to 0.3 times (p=0.0397). The expression of BMP2 in cells treated with miR-214 mimics was significantly increased to 2.5-fold (p=0.003), while the expression of BMP2 was significantly decreased in cells treated with miR-214 AMO, reduced to 0.3 times (p=0.0002). miR-214 can regulate the expression of Sox2, PI3K and AKT proteins.
Conclusion:
MiR-214 regulates the proliferation, apoptosis, bone formation of osteoblasts and participate in the fracture healing process by inhibiting the expression of Sox4, which provided new ideas for clinical treatment of fracture healing.
Insights
Micro ribonucleic acid (miR)-214 expression declines in patients with fragility fractures. This microRNA promotes osteoblast proliferation and bone healing by inhibiting Sox4, offering new therapeutic avenues.
Area of Science:
- Molecular Biology
- Biochemistry
- Orthopedics
Background:
- Fragility fractures represent a significant clinical challenge.
- MicroRNAs (miRNAs) play crucial roles in cellular processes and disease.
- Understanding the role of specific miRNAs in fracture healing is essential for developing novel treatments.
Purpose of the Study:
- To investigate the expression levels of micro ribonucleic acid (miR)-214 in bone and blood samples from patients with fragility fractures.
- To elucidate the functional role of miR-214 in osteoblast proliferation, apoptosis, and fracture healing.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to measure miR-214 expression.
- Osteoblast proliferation was assessed using the MTT assay.
- Apoptosis was evaluated via TUNEL staining.
- Protein expression of BSP, BMP2, Sox4, PI3K, and AKT was analyzed.
Main Results:
- miR-214 expression was significantly decreased in the bone tissue and blood of patients with fragility fractures.
- miR-214 promoted osteoblast proliferation and inhibited apoptosis.
- miR-214 enhanced the expression of bone sialoprotein (BSP) and bone morphogenetic protein 2 (BMP2).
- miR-214 was shown to inhibit Sox4 expression and promote the phosphorylation of the PI3K/AKT pathway.
Conclusions:
- miR-214 plays a critical role in regulating osteoblast function and fracture healing.
- The findings suggest that miR-214 influences fracture healing by modulating osteoblast proliferation, apoptosis, and bone formation.
- Targeting miR-214 presents a potential therapeutic strategy for improving fracture healing in patients with fragility fractures.
