Hsa-miR-30e-3p inhibits influenza B virus replication by targeting viral NA and NP genes
Kritsada Khongnomnan1,2, Suthat Saengchoowong1, Oraphan Mayuramart3
1Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Abstract:
Influenza B virus is a member of the Orthomyxoviridae family which can infect humans and causes influenza. Although it is not pandemic like influenza A virus, it nevertheless affects millions of people worldwide annually. MicroRNAs are small non-coding RNAs regulating gene expression at posttranscriptional level. They play various important roles in cellular processes including response to viral infection. MiRNA profiles from our previous study suggested that miR-30e-3p was one of the upregulated miRNAs that responded to influenza B virus infection. In this study, in silico prediction and in vitro investigation proved that this miRNA can directly target NA and NP genes of the influenza B virus and inhibit its replication. This finding might be useful for using miRNA as an alternative therapeutics for influenza virus infection.
Insights
MicroRNAs, like miR-30e-3p, can target influenza B virus genes to inhibit replication. This discovery offers potential for novel miRNA-based therapeutics against influenza B virus infection.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Influenza B virus, an Orthomyxoviridae family member, causes annual global infections.
- MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression and cellular responses to viral infections.
- Previous studies indicated miR-30e-3p upregulation in response to influenza B virus.
Purpose of the Study:
- To investigate the role of miR-30e-3p in influenza B virus infection.
- To determine if miR-30e-3p directly targets influenza B virus genes.
- To assess the potential of miR-30e-3p as a therapeutic agent against influenza B virus.
Main Methods:
- In silico prediction analysis to identify potential miRNA targets.
- In vitro experiments to validate miRNA-target interactions.
- Replication assays to measure the effect of miR-30e-3p on influenza B virus.
Main Results:
- miR-30e-3p was confirmed to directly target the NA and NP genes of influenza B virus.
- Overexpression of miR-30e-3p significantly inhibited influenza B virus replication.
- In silico and in vitro data supported the inhibitory role of miR-30e-3p.
Conclusions:
- miR-30e-3p directly targets and inhibits the replication of influenza B virus.
- This miRNA holds promise as a potential therapeutic strategy for influenza B virus infections.
- Further research into miRNA-based therapies for influenza is warranted.
More Related Videos
11:20Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
12:18Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
Published on: November 6, 2013
