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Published on: January 22, 2019
Luteolin Induces Cytotoxicity in Mix Cellularity Classical Hodgkin's Lymphoma via Caspase Activated-cell Death
Rajendra Gharbaran1,2, Enyuan Shang3, Onyekwere Onwumere2,4
1Department of Biological Sciences, Bronx Community College/The City University of New York, Bronx, NY, U.S.A. rajendra.gharbaran@bcc.cuny.edu.
Background/Aim:
We investigated the effects of luteolin (LUT) on classical Hodgkin's lymphoma (cHL), since such studies in malignant lymphomas are lacking.
Materials And Methods:
Effect of LUT on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry on trypan blue-exclusion assay. Cell death was investigated with acridine orange/ethidium bromide live-dead assay, propidium iodide (PI) flow cytometry, and Annexin-V-PI microscopy. Caspase activation was studied using CellEvent Caspase-3/7 Green detection reagent. High resolution immunofluorescence microscopy was used to detect cleaved-PARP-1.
Results:
LUT induced a dose-dependent decrease in the growth of KMH2 and L428 cells, cellular models of mix-cellularity (MC) and nodular sclerosis (NS) cHL, respectively. However, LUT induced cell death only in KMH2, at a higher concentration, and this was associated with caspase activation and cleaved PARP-1.
Conclusion:
LUT induces cytotoxicity in the MC-cHL cellular model KMH2 via caspase activation.
