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Related Experiment Video

Updated: Dec 10, 2025

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes
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Cardiac hypertrophy in a dish: a human stem cell based model.

Markus Johansson1,2, Benjamin Ulfenborg3, Christian X Andersson4

  • 1Systems Biology Research Center, School of Bioscience, Department for Biology and Bioinformatics, University of Skövde, SE-541 28 Skövde, Sweden markus.johansson@his.se.

Biology Open
|September 4, 2020
PubMed
Summary

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) exposed to endothelin-1 (ET-1) showed hallmarks of cardiac hypertrophy. This study provides a valuable in vitro model for understanding cardiac hypertrophy mechanisms and developing new therapies.

Keywords:
Cardiac hypertrophyCardiomyocytesDisease modelEndothelin-1Stem cells

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Area of Science:

  • Cardiovascular Biology
  • Stem Cell Biology
  • Molecular Cardiology

Background:

  • Cardiac hypertrophy is a significant risk factor for heart failure.
  • Improved in vitro models are needed to study cardiac hypertrophy mechanisms.
  • Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer a promising model system.

Purpose of the Study:

  • To investigate the hypertrophic response of hiPSC-CMs upon stimulation with endothelin-1 (ET-1).
  • To analyze key cellular and molecular changes indicative of cardiac hypertrophy.
  • To identify gene expression patterns associated with ET-1-induced hypertrophy.

Main Methods:

  • hiPSC-CMs were stimulated with ET-1 for varying durations (8-96 hours).
  • Measurements included cell size, ANP, proBNP (protein and mRNA), and lactate concentration.
  • Transcriptional profiling was performed using RNA-sequencing (RNA-seq).

Main Results:

  • ET-1 stimulation led to a ~13% increase in hiPSC-CM size.
  • Increased ANP and proBNP levels (protein and mRNA) were observed.
  • Elevated lactate concentration indicated increased glucose consumption, a hallmark of hypertrophy.
  • RNA-seq revealed a distinct hypertrophic gene expression pattern.

Conclusions:

  • ET-1 effectively induces a hypertrophic response in hiPSC-CMs.
  • This model provides new molecular insights into cardiac hypertrophy.
  • The findings may aid in developing novel therapeutic strategies for cardiac hypertrophy and heart failure.