Synergistic Antimicrobial Titanium Carbide (MXene) Conjugated with Gold Nanoclusters

Bacterial resistance toward antibiotics is a world-wide problem, and one potential solution to fight against the resistance is to develop multi-mechanism antimicrobial agents to achieve synergistic performance. Titanium carbide (MXene) is an emerging 2D nanomaterial with antimicrobial ability to physically damage bacterial membrane and chemically induce oxidative stress, and it can be further conjugated with nanomaterials to improve its antibacterial performance. Herein, a synergistic antimicrobial agent is developed through conjugation of the ultra-small gold nanoclusters (AuNCs) on MXene nanosheets. The conjugated AuNCs are effectively delivered into bacteria after bacterial membrane damage caused by MXene, generating localized reactive oxygen species (ROS) of high concentration to effectively oxidize bacterial membrane lipid for enhanced membrane broken, as well as bacterial DNA for violent fragmentation. Thus, the synergistic physical (via MXene) and chemical (via MXene and AuNCs) antimicrobial mechanisms lead to eventual bacterial death of both Gram-positive and Gram-negative bacteria, with low IC₅₀ values of 11.7 µg mL^-1 of MXene and 0.04 µm of AuNCs. Moreover, the crumpled MXene-AuNCs structure is constructed to inhibit biofilm formation, which hold synergistic antibacterial ability of MXene-AuNCs conjugation, hydrophobic surface to prevent bacterial attachment, and large surface area containing higher density of bactericides.