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The draft genome of Labeo catla
Lakshman Sahoo1, Paramananda Das2, Bismay Sahoo1
1Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, Odisha, 751002, India.
BMC Research Notes
|September 5, 2020
Summary
Researchers have generated the first draft genome assembly for Labeo catla (catla), a popular aquaculture fish. This genomic resource will aid in the genetic improvement of catla for enhanced aquaculture practices.
Area of Science:
- Aquaculture genomics
- Fish genetics
- Comparative genomics
Background:
- Labeo catla (catla) is a key species in Indian aquaculture due to its rapid growth and consumer preference.
- Genomic resources for catla are currently limited, hindering genetic improvement efforts.
- Advancements in sequencing technologies enable the development of genomic tools for aquaculture species.
Purpose of the Study:
- To perform the first de-novo genome assembly of Labeo catla.
- To provide foundational genomic resources for catla aquaculture.
- To facilitate future genetic studies and breeding programs for catla.
Main Methods:
- High molecular weight genomic DNA was extracted from a male catla.
- DNA was sequenced using Oxford Nanopore and Illumina platforms.
- A hybrid assembly strategy was employed to assemble the genome data.
Main Results:
- The de-novo assembly resulted in a 1.01 Gb genome size with 5345 scaffolds.
- The assembly achieved an N50 value of 0.7 Mb and over 92% BUSCO completeness.
- Gene annotation identified 25,812 predicted genes.
Conclusions:
- The first draft genome assembly of Labeo catla has been successfully generated.
- This genomic resource is crucial for advancing genetic improvement in catla aquaculture.
- The study provides essential data for future research in fish genomics and breeding.
