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Updated: Dec 10, 2025

The Organoid Reconstitution Assay ORA for the Functional Analysis of Intestinal Stem and Niche Cells
Published on: November 20, 2017
Cellular and molecular architecture of the intestinal stem cell niche
Neil McCarthy1,2, Judith Kraiczy1,2, Ramesh A Shivdasani3,4
1Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Abstract:
Intestinal stem and progenitor cells replicate and differentiate in distinct compartments, influenced by Wnt, BMP, and other subepithelial cues. The cellular sources of these signals were long obscure because intestinal mesenchyme was insufficiently characterised. In this Review, we discuss how recent mRNA profiles of mouse and human intestinal submucosa, coupled with fine-resolution microscopy and gene and cell disruptions, reveal a coherent picture of an organised tissue carrying cells with distinct molecular properties and functions.
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