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miR-133b Suppresses Invasion and Migration of Gastric Cancer Cells via the COL1A1/TGF-β Axis

Yuan Guo1, Guochun Lu1, Huahui Mao1

  • 1Department of General Surgery, First People's Hospital of Tonglu, Hangzhou 311500, People's Republic of China.

Oncotargets and Therapy
|September 5, 2020
PubMed
Abstract

Insights

MicroRNA-133b (miR-133b) inhibits gastric cancer (GC) cell invasion and migration by targeting COL1A1 and suppressing the TGF-β pathway. This finding offers new avenues for GC diagnosis and targeted therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) remains a significant global health challenge.
  • Understanding the molecular mechanisms underlying GC cell invasion and migration is crucial for developing effective therapies.

Purpose of the Study:

  • To elucidate the regulatory role of miR-133b in gastric cancer cell invasion and migration.
  • To investigate the involvement of the COL1A1/TGF-β signaling axis in miR-133b-mediated GC progression.

Main Methods:

  • Bioinformatic analysis of TCGA database for miRNA and gene expression profiling.
  • Quantitative real-time PCR (qRT-PCR) to assess gene expression levels.
  • Cellular assays including CCK-8, wound healing, and Transwell assays to evaluate cell proliferation, migration, and invasion.
  • Western blot analysis for epithelial-mesenchymal transition (EMT) markers.
  • Dual-luciferase reporter assays to confirm gene interactions.

Main Results:

  • miR-133b was significantly downregulated in GC tissues, while COL1A1 expression was upregulated.
  • Overexpression of miR-133b inhibited GC cell invasion, migration, and EMT, whereas silencing miR-133b had opposite effects.
  • COL1A1 was identified as a direct target gene of miR-133b.
  • COL1A1 was enriched in the TGF-β signaling pathway, and its activation promoted GC cell proliferation and migration.
  • miR-133b overexpression suppressed the TGF-β pathway, and simultaneous overexpression of miR-133b and COL1A1 reversed the inhibitory effects.

Conclusions:

  • miR-133b acts as a tumor suppressor in gastric cancer by inhibiting cell invasion and migration through the COL1A1/TGF-β axis.
  • This study provides novel insights into the molecular pathogenesis of GC.
  • The miR-133b/COL1A1/TGF-β pathway represents a potential therapeutic target for gastric cancer treatment.
Keywords:
COL1A1TGF-β signaling pathwaygastric cancerinvasion and migrationmiR-133b

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