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Updated: Dec 10, 2025
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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
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Methodological approaches for vitrification of bovine oocytes
Linda Dujíčková1,2, Alexander V Makarevich1, Lucia Olexiková1
1National Agricultural and Food Centre (NPPC), Research Institute for Animal Production Nitra, Hlohovecká 2, 95141Lužianky-near-Nitra, Slovak Republic.
Zygote (Cambridge, England)
|September 5, 2020
Summary
Optimizing bovine oocyte vitrification is crucial for successful cryopreservation and embryo development. This review examines key factors influencing oocyte cryotolerance and post-thaw recovery for improved in vitro fertilization outcomes.
Area of Science:
- Reproductive Biology
- Cryobiology
- Embryology
Background:
- Vitrification success and post-thaw oocyte development are influenced by numerous factors.
- Optimal methodologies for higher oocyte cryotolerance and blastocyst yield are of significant interest.
Purpose of the Study:
- To describe and evaluate critical factors affecting oocyte vitrification success.
- To review methodologies for cryopreservation and post-thaw oocyte development in bovine species.
Main Methods:
- Evaluation of oocyte maturation status (germinal vesicle, metaphase II).
- Assessment of cumulus cell presence, follicle size, and cryoprotectant effects.
- Analysis of cooling/warming rates, cryocarriers, and donor age (heifers, cows).
Main Results:
- Specific oocyte stages, cumulus cell presence, and follicle size impact vitrification success.
- Cryoprotectant type/concentration, cooling/warming rates, and cryocarriers are critical.
- Donor age influences post-thaw oocyte development.
Conclusions:
- Optimizing bovine oocyte vitrification requires careful consideration of multiple factors.
- Improved cryopreservation techniques enhance oocyte cryotolerance and subsequent embryo development.
- This review provides insights into successful bovine oocyte cryopreservation methodologies.
