Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cosolutes Modulate Polyubiquitin Fibrillation.

ACS omega·2026
Same author

In Memoriam: Robert M. Kelly (1953-2026).

Applied and environmental microbiology·2026
Same author

SILAC-based discovery of a broad range of proteins that can be posttranslationally glutamylated or glycylated.

Journal of cell science·2026
Same author

In Memoriam: Robert M. Kelly (1953-2026).

Applied and environmental microbiology·2026
Same author

Tubulin hyperglutamylation induces retinal degeneration by disrupting ciliary architecture and protein trafficking.

Zoological research·2026
Same author

Tubulin hyperglutamylation causes retinal degeneration by impairing the structural integrity of cilia and protein mistrafficking.

Zoological research·2026

Related Experiment Video

Updated: Jun 20, 2026

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
10:23

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Published on: April 20, 2017

9.8K

Genetically encoded live-cell sensor for tyrosinated microtubules.

Shubham Kesarwani1,2, Prakash Lama1,2, Anchal Chandra3

  • 1Centre for Cardiovascular Biology and Diseases, Institute for Stem Cell Science and Regenerative Medicine, Gandhi Krishi Vigyan Kendra Campus, Bangalore, India.

The Journal of Cell Biology
|September 5, 2020
PubMed
Summary

Researchers developed a novel nanobody sensor to track tyrosinated microtubules in living cells. This tool allows real-time observation of microtubule dynamics and posttranslational modifications (PTMs).

More Related Videos

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

3.3K
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

454

Related Experiment Videos

Last Updated: Jun 20, 2026

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
10:23

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Published on: April 20, 2017

9.8K
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

3.3K
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

454

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Biochemistry

Background:

  • Microtubule cytoskeleton exhibits diverse biochemical forms due to tubulin posttranslational modifications (PTMs).
  • Tubulin PTMs influence microtubule stability, dynamics, and interactions, forming the basis of the 'tubulin code hypothesis'.
  • Current limitations exist in tools for specifically marking tubulin PTMs in living cells, hindering research on their dynamics and functions.

Purpose of the Study:

  • To develop a novel tool for specifically marking and studying tubulin PTMs in living cells.
  • To create a live-cell nanobody sensor for tyrosinated microtubules, a specific tubulin PTM.
  • To investigate the real-time effects of depolymerizing agents on tyrosinated microtubules.

Main Methods:

  • Utilized a yeast display library to identify a specific binder against terminal tyrosine of α-tubulin.
  • Characterized the binder's robustness and nonperturbing nature as a tyrosination sensor.
  • Employed the sensor to monitor nocodazole-, colchicine-, and vincristine-induced microtubule depolymerization in real time.

Main Results:

  • Successfully identified and validated a nanobody specific for tyrosinated microtubules, functioning as a live-cell tyrosination sensor.
  • Demonstrated the sensor's nonperturbing nature, allowing for observation without interfering with cellular processes.
  • Observed distinct real-time depolymerization patterns of tyrosinated microtubules induced by different agents (nocodazole, colchicine, vincristine).

Conclusions:

  • A novel tyrosination sensor (nanobody) has been developed for live-cell imaging of tyrosinated microtubules.
  • This sensor enables real-time studies of microtubule dynamics and the impact of PTMs.
  • The findings highlight the potential of this sensor for advancing the understanding of microtubule PTMs and cellular functions.