Roseomonas bella sp. nov., isolated from lake sediment

A Gram-stain-negative, non-motile, coccus-shaped, catalase- and oxidase-positive, facultatively anaerobic and pink-pigmented bacterium, designated strain CQN31^T, was isolated from sediment of Changqiaohai Lake, Yunnan Province, China. Growth occurred at 4-45 °C (optimum, 37 °C), at pH 6.5-9.5 (optimum, pH 8.0) and with 0-1 % (w/v) NaCl (optimum, 0 %). C_18 : 1 ω7c/C_18 : 1 ω6c and C_16 : 0 were the predominant fatty acids. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidyldimethylethanolamine (PME) and one unidentified aminolipid (AL) were the major polar lipids. The G+C content of the genomic DNA was 71.5 %. 16S rRNA gene sequence comparisons indicated that strain CQN31^T shared 96.8 % similarity with Roseomonas wooponensis JCM 19527^T and 95.9 % with R. terricola EM0302^T. Digital DNA-DNA hybridization values between strain CQN31^T and Roseomonas stagni DSM 19981^T, R. rosea DSM 14916^T and R. mucosa NCTC 13291^T were 21.0, 19.4 and 19.8 %, respectively. Average amino acid identity and average nucleotide identity values between strain CQN31^T and R. stagni DSM 19981^T, R. rosea DSM 14916^T and R. mucosa NCTC 13291^T were 73.7, 63.4 and 61.9 %, and 79.2, 77.1 and 77.5%, respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain CQN31^T as a representative of a novel species in the genus Roseomonas, for which the name Roseomonas bella sp. nov. is proposed. The type strain is CQN31^T (=KCTC 62447^T=MCCC 1H00309^T).