Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Detection of Leishmania RNA virus 2 in clinical isolates of Leishmania from Qom Province, Iran.

Archives of virology·2025

Same author

Comparative gene expression pattern of immune-related genes using dual-color RT-MLPA in the lesions of cutaneous leishmaniasis caused by L. major and L. tropica.

PLoS neglected tropical diseases·2025

Same author

Guidelines for the purification and characterization of extracellular vesicles of parasites.

Journal of extracellular biology·2024

Same author

Cutaneous leishmaniasis: multiomics approaches to unravel the role of immune cells checkpoints.

Expert review of proteomics·2022

Same author

Computational evaluation of modified peptides from human neutrophil peptide 1 (HNP-1).

Journal of biomolecular structure & dynamics·2020

Same author

A Synthetic Peptide 2Abz<sup>23</sup>S<sup>29</sup> Reduces Bacterial Titer and Induces Pro-Inflammatory Cytokines in a Murine Model of Urinary Tract Infection.

Drug design, development and therapy·2020

Same journal

Plasma proteome profiles of standard-risk and high-risk childhood acute lymphoblastic leukemia.

Expert review of proteomics·2026

Same journal

Potential proteomic biomarkers for monitoring clinical studies in Duchenne/Becker muscular dystrophy.

Expert review of proteomics·2026

Same journal

Proteomics-based approaches to neutrophil biology.

Expert review of proteomics·2026

Same journal

Deciphering mitochondrial metabolic vulnerabilities in ovarian clear cell carcinoma with mass spectrometry-based clinical proteomics.

Expert review of proteomics·2026

Same journal

The proteomic origin of the genetic code.

Expert review of proteomics·2026

Same journal

Toward large-scale mass spectrometry-based omics for clinical applications.

Expert review of proteomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 10, 2025

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

Transcriptomic profiling in Cutaneous Leishmaniasis patients.

Nasrin Masoudzadeh¹, Amir Mizbani², Sima Rafati¹

¹Department of Immunotherapy and Leishmania Vaccine Research, Pasteur Institute of Iran , Tehran, Iran.

Expert Review of Proteomics

|September 4, 2020

Summary

This summary is machine-generated.

This review explores transcriptomics in human cutaneous leishmaniasis (CL) patients, analyzing host-pathogen interactions. It highlights how gene expression studies can identify biomarkers for CL research and vaccine development.

Keywords:

Cutaneous leishmaniasis human skin lesion microarray rna-seq transcriptomics

More Related Videos

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Published on: July 27, 2010

Related Experiment Videos

Last Updated: Dec 10, 2025

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Published on: July 27, 2010

Area of Science:

Immunology
Genomics
Parasitology

Background:

Cutaneous leishmaniasis (CL) involves parasite-induced skin inflammation and ulceration.
Most CL research uses mouse models, leaving human transcriptional responses understudied.
Human studies offer direct insight into host-pathogen interactions at the lesion site.

Purpose of the Study:

To review transcriptomics studies in human CL patients.
To characterize the host-pathogen molecular interplay in CL.
To identify novel CL biomarkers for research and vaccine development.

Main Methods:

Analysis of microarray and RNA-seq data from CL patient blood and tissue samples.
Application of systems biology approaches to whole genome analysis.
Review of existing literature on human CL transcriptomics.

Main Results:

Transcriptomics reveals crucial molecular interactions between Leishmania parasites and human hosts.
Identification of potential biomarkers for CL diagnosis and prognosis.
Insights into immune responses driving CL pathogenesis.

Conclusions:

Transcriptomics is a powerful tool for understanding human CL.
Identified biomarkers can advance CL research and accelerate vaccine development.
Further research integrating transcriptomics and systems biology is warranted for CL management.