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Induction of Cell Cycle Arrest in MKN45 Cells after Schiff Base Oxovanadium Complex Treatment Using Changes in Gene

Sara Mirjalili1, Maedeh Dejamfekr1, Abdolvahab Moshtaghian2

  • 1Department of Biochemistry, Semnan University of Medical Sciences, Semnan, Iran.

Drug Research
|September 4, 2020
PubMed

Insights

Vanadium compounds significantly inhibit gastric cancer cell growth and migration. The schiff base oxovanadium complex induces cell cycle arrest at the G2/M phase, offering potential for new cancer therapies.

Area of Science:

  • Inorganic Chemistry
  • Medicinal Chemistry
  • Cancer Biology

Background:

  • Heavy metal compounds, including vanadium, nickel, and cobalt, show promise for disease treatment.
  • Vanadium-containing compounds have demonstrated anticancer effects across various cell types.
  • Gastric cancer remains a significant health concern, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To investigate the effects of a specific schiff base oxovanadium complex on gastric cancer cells (MKN45).
  • To evaluate the complex's impact on cell cycle arrest, proliferation, and migration.
  • To analyze the modulation of key genes involved in cell division (P53, GADD45, CDC25).

Main Methods:

  • Cytotoxicity was assessed using cell viability assays.
  • Cell cycle phase distribution was analyzed via propidium iodide (PI) staining and flow cytometry.
  • Gene expression changes for P53, GADD45, and CDC25 were quantified.

Main Results:

  • The schiff base oxovanadium complex significantly reduced MKN45 cell viability in a time- and concentration-dependent manner.
  • Cancer cell migration and metastatic activities were inhibited by the complex.
  • Cell cycle arrest at the G2/M phase was observed after 48 hours of treatment at 1 μg/ml.
  • This arrest correlated with increased expression of P53 and GADD45, and decreased expression of CDC25.

Conclusions:

  • Schiff base oxovanadium complex effectively inhibits the growth, proliferation, and migration of MKN45 gastric cancer cells.
  • The complex induces G2/M phase cell cycle arrest, likely through DNA damage-mediated activation of cell division controllers.
  • These findings highlight the therapeutic potential of vanadium-based compounds in gastric cancer treatment.

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