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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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A dual-function oligonucleotide-based ratiometric fluorescence sensor for ATP detection.

Yao-Yao Fan1, Xu Deng1, Man Wang1

  • 1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.

Talanta
|September 5, 2020
PubMed
Summary
This summary is machine-generated.

A novel ratiometric fluorescence aptasensor was developed for sensitive adenosine triphosphate (ATP) detection. This method utilizes a dual-function DNA strand and offers accurate ATP quantification in biological samples.

Keywords:
Adenosine triphosphateAptasensorDual-function DNARatiometric fluorescence

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Adenosine triphosphate (ATP) is crucial for physiological processes, necessitating precise detection methods.
  • Current diagnostic and research applications require sensitive and selective ATP quantification techniques.

Purpose of the Study:

  • To develop a ratiometric fluorescence aptasensor for accurate and sensitive detection of ATP.
  • To create a sensor utilizing a dual-function DNA strand for enhanced detection capabilities.

Main Methods:

  • Designed and optimized a dual-function DNA strand with ATP-binding aptamer (ABA) and berberine-binding aptamer (BBA).
  • Utilized the specific binding of ATP to ABA, causing Thioflavin T (ThT) release and fluorescence quenching.
  • Employed berberine fluorescence as a stable reference signal for ratiometric analysis.

Main Results:

  • The aptasensor demonstrated excellent selectivity and high sensitivity for ATP detection, with a limit of detection as low as 24.8 nM.
  • ATP binding to ABA induced a measurable decrease in ThT fluorescence, while berberine fluorescence remained stable.
  • The ratiometric approach provided reliable ATP quantification, unaffected by variations in berberine-DNA interaction.

Conclusions:

  • The developed ratiometric fluorescence aptasensor offers a robust platform for sensitive and selective ATP detection.
  • The method shows promise for practical applications in biological samples, including human serum and urine.
  • This innovative sensor design provides a valuable tool for biochemical research and clinical diagnostics.