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Updated: Dec 10, 2025
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Engineering of a dual-site molecular probe for logical bioimaging of lysosomal H2S and pH
Yongchao Liu1, Yingjun Zhang2, Xiao-Bing Zhang3
1State Key Laboratory of Anti-Infective Drug Development, Dongguan, 523871, China; Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.
Abstract:
Lysosomes contains numerous enzymes and proteins closely linked with cellular metabolism. The variation of lysosomal pH is closely related to cell homeostasis while hydrogen sulfide (H2S) has been regarded as an important gasotransmitter. Herein, via rational design, a high-sensitivity fluorescent probe ANp-Rh-Lys was synthesized for logical detection and bioimaging of H2S and pH in lysosomes of living cells. The probe showed different fluorescence signals towards H2S and lysosomal pH. Significantly, ANp-Rh-Lys is membrane-permeable and suitable for visualization of both pH variations and endogenous H2S in lysosomes. This logical design strategy may have great potential for detection of multiple analytes in complicated biological systems.
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