Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.4K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.4K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.8K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.7K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytokinin histidine kinase receptors regulate multiple aspects of rice growth and development.

Plant physiology·2026
Same author

1-Aminocyclopropane-1-carboxylic acid oxidase determines the fate of ethylene biosynthesis in a tissue-specific way †.

Plant physiology·2026
Same author

Platelet rich plasma augmentation for meniscus repair reduces failure but not complication rates or outcomes: A systematic review and meta-analysis.

Journal of orthopaedics·2026
Same author

Ulnar collateral ligament repair and reconstruction have similar return to sport rates with low complication rates: a systematic review and meta-analysis.

JSES reviews, reports, and techniques·2025
Same author

A Case of Steroid-Dependent Immune Checkpoint Inhibitor Pneumonitis Treated With Subcutaneous Tocilizumab.

Respirology case reports·2025
Same author

Pembrolizumab-Exacerbated Widespread Pigmented Purpuric Dermatosis in an Elderly Patient, a Potential Diagnostic Pitfall Mimicking Pigmented Purpuric Dermatosis-Like Mycosis Fungoides.

Journal of cutaneous pathology·2025

Related Experiment Video

Updated: Dec 10, 2025

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.5K

Mutagenomics: A Rapid, High-Throughput Method to Identify Causative Mutations from a Genetic Screen.

Charles Hodgens1,2, Nicole Chang1, G Eric Schaller3

  • 1Department of Biology, University of North Carolina at Chapel Hill, North Carolina 27599.

Plant Physiology
|September 5, 2020
PubMed
Summary

Mutagenomics accelerates gene discovery in genetic screens by analyzing mutant genomes. This high-throughput method efficiently identifies causal mutations without backcrossing, enhancing Arabidopsis research.

More Related Videos

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

11.2K
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
04:51

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

9.6K

Related Experiment Videos

Last Updated: Dec 10, 2025

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.5K
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

11.2K
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
04:51

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

9.6K

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Genetic screens are vital for understanding biological processes.
  • Identifying causative genes after screening is often a bottleneck.

Purpose of the Study:

  • To introduce
  • mutagenomics,
  • a high-throughput strategy for identifying causal mutations from genetic screens.
  • To demonstrate the utility of mutagenomics in Arabidopsis research.

Main Methods:

  • Genomic sequencing of mutants followed by a three-stage analysis pipeline.
  • Stage 1: Identifies sequence changes in known pathway genes.
  • Stage 2: Uses simulations to find genes with multiple independent alleles.
  • Stage 3: Sequences sibling lines to identify candidate genes for remaining mutants.

Main Results:

  • Mutagenomics successfully identified causative genes in an Arabidopsis screen for cytokinin response mutants.
  • Identified 13 alleles of the ARABIDOPSIS HIS KINASE4 gene.
  • Discovered mutations in 1-AMINOCYCLOPROPANE-1-CARBOXYLATE (ACC) SYNTHASE7 and ELONGATED HYPOCOTYL5 (HY5).
  • HY5 was found to regulate a portion of the cytokinin transcriptional response.

Conclusions:

  • Mutagenomics offers a rapid and efficient method for identifying causal mutations in genetic screens.
  • This approach can significantly accelerate the pace and broaden the applicability of genetic screens in model organisms like Arabidopsis.