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Resveratrol: Friend or Foe?
Thomas L Clarke1, Raul Mostoslavsky2
1The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02114, USA.
Molecular Cell
|September 5, 2020
Summary
Resveratrol and Pterostilbene induce DNA replication stress in mammalian cells. A CRISPR-Cas9 screen identified key genes involved in DNA replication and genome integrity affected by these compounds.
Area of Science:
- Molecular Biology
- Genetics
- Pharmacology
Background:
- Nutraceutical compounds like Resveratrol and Pterostilbene are widely studied for their health benefits.
- The precise molecular mechanisms underlying their effects, particularly on cellular processes like DNA replication, remain incompletely understood.
Purpose of the Study:
- To investigate the effects of Resveratrol and Pterostilbene on DNA replication and genome integrity in mammalian cells.
- To identify specific genes and pathways modulated by these compounds using a chemogenomic approach.
Main Methods:
- Utilized a CRISPR-Cas9 whole-genome screen for chemogenomic profiling.
- Assessed the impact of Resveratrol and Pterostilbene on DNA replication stress markers.
- Analyzed the network of genes associated with DNA replication and genome integrity.
Main Results:
- Identified a network of genes critical for DNA replication and genome integrity.
- Demonstrated that Resveratrol and Pterostilbene induce DNA replication stress.
- Linked these nutraceuticals to specific genetic pathways involved in maintaining genomic stability.
Conclusions:
- Resveratrol and Pterostilbene trigger DNA replication stress in mammalian cells.
- The study elucidates a genetic network sensitive to these compounds, highlighting their role in genome integrity maintenance.
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