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Updated: Dec 9, 2025
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Proteomic analysis of wheat seeds produced under different nitrogen levels before and after germination
Daxing Wen1, Haicheng Xu1, Mingrong He1
1State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong Province 271018, PR China.
Abstract:
The objective of this study was to investigate differentially abundant proteins (DAPs) of wheat seeds produced under two nitrogen levels (0 and 240 kg/ha) before and after germination. We selected samples at 8 and 72 h after imbibition (HAI) to identify DAPs by iTRAQ. The results showed 190 and 124 DAPs at 8 and 72 HAI, respectively. Alpha-gliadin and chlorophyll a-b binding protein showed the biggest difference in abundance before and after germination. In GO enrichment analysis, the most significantly enriched GO term was nutrient reservoir activity at 8 HAI and endopeptidase inhibitor activity at 72 HAI. Moreover, many DAPs involved in mobilization of stored nutrients and photosynthesis were mapped to KEGG pathways. Dough development time, dough stability time and seedling chlorophyll content under N240 were significantly higher than those under N0, which validated the results of proteomic analysis. These results are crucial for food nutrition and food processing.
