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Use of Yeast Plasmids: Transformation and Inheritance Assays
Anastasiia Mereshchuk1, Joyce S K Chew1, Melanie J Dobson2
1Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, NS, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|September 5, 2020
Summary
This study introduces yeast shuttle vectors for genetic research. It covers low- and high-copy plasmids, selection markers, transformation methods, and inheritance monitoring in Saccharomyces cerevisiae.
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- The budding yeast Saccharomyces cerevisiae is a crucial model organism in genetic research.
- Efficient genetic manipulation relies on versatile plasmid vectors.
- Yeast shuttle vectors, replicable in E. coli and yeast, are essential tools.
Purpose of the Study:
- To provide a comprehensive introduction to yeast shuttle vectors.
- To detail the types, selection, and application of plasmids in Saccharomyces cerevisiae.
Main Methods:
- Description of low-copy (ARS/CEN) and multi-copy (2-μm-based) plasmid systems.
- Overview of common marker genes for yeast plasmid selection.
- Explanation of yeast transformation techniques and plasmid inheritance monitoring.
Main Results:
- Availability of diverse yeast shuttle vectors enables stable plasmid maintenance.
- Established methods facilitate yeast transformation and tracking of plasmid inheritance.
- Practical tips are provided for working with yeast transformants.
Conclusions:
- Yeast shuttle vectors significantly advance Saccharomyces cerevisiae as a model genetic organism.
- Understanding plasmid systems, selection, and transformation is key for successful yeast genetics.
