Visualizing NH₃ emission and the local O₂ and pH microenvironment of soil upon manure application using optical...

The application of fertilizers and manure on fields is the largest source of ammonia (NH₃) in the atmosphere.·NH₃ emission from agriculture has negative environmental consequences and is largely controlled by the chemical microenvironment and the respective biological activity of the soil. While gas phase and bulk measurements can describe the emission on a large scale, those measurements fail to unravel the local processes and spatial heterogeneity at the soil air interface. We report a two dimensional (2D) imaging approach capable of visualizing three of the most important chemical parameters associated with NH₃ emission from soil. Besides the released NH₃ itself also O₂ and pH microenvironments are imaged using reversible optodes in real-time with a spatial resolution of <100 µm. This combined optode approach utilizes a specifically developed NH₃ optode with a limit of detection of 2.11 ppm and a large working range (0-1800 ppm) ideally suited for studying NH₃ volatilization from soil. This NH₃ optode will contribute to a better understanding of the driving factors for NH₃ emission on a microscale and has the potential to become a valuable tool in studying NH₃ dynamics.