Photonic crystal surface mode imaging for multiplexed and high-throughput label-free biosensing

Area of Science:

• Biophotonics
• Biosensing Technology
• Surface Chemistry

Background:

• Label-free biosensing is crucial for real-time monitoring of biomolecular interactions.
• Existing techniques like surface plasmon resonance imaging (SPRi) offer high sensitivity but can be limited in throughput.
• Simultaneous detection of multiple analytes in a single assay is essential for high-throughput screening.

Purpose of the Study:

• To implement and evaluate a photonic crystal surface mode imaging (PCSMi) technique for simultaneous, label-free detection of antibody-antigen binding.
• To demonstrate the capability of PCSMi for high-density arrays (96- and 384-spots).
• To assess the kinetic recording capabilities and resolution of the PCSMi technique.

Main Methods:

• Photonic crystal surface mode imaging (PCSMi) was employed for label-free detection.
• The technique was applied to arrays with 96- and 384-spots for antibody-antigen interactions.
• Adsorption kinetics were recorded simultaneously across all spots with high resolution.

Main Results:

• Simultaneous detection of antibody binding to specific antigens was achieved in high-density arrays.
• The PCSMi technique demonstrated sub-picogram resolution in adsorption kinetics measurements.
• The method allows interrogation of analytes by hundreds of immobilized ligands.

Conclusions:

• The PCSMi technique is a viable label-free, high-throughput method for biosensing.
• It offers simultaneous kinetic analysis with high sensitivity and spatial resolution.
• Potential applications include multiplexed and high-throughput screening in diagnostics and drug discovery.