Related Experiment Video
Updated: Dec 9, 2025
Author Spotlight: In Vivo Assessment of Thyroid Hormone Disruption Using the THAI Mouse Model
Published on: October 6, 2023
[Thyroid disruptor p, p'-DDE inhibited the expression of LHX4 and DIS3L protein in Nthy-ori-3-1 cells]
Abstract:
Objective: To observe the changes of LHX4 and DIS3L mRNA and protein expression in Nthy-ori-3-1 cells after the treatment of thyroid disruptor p, p'-DDE. Methods: Nthy-ori-3-1 cells in logarithmic growth phase were treated with 0, 0.5, 1.0, 2.0 and 5.0 μg/ml p, p'-DDE solution. The growth state and morphology of the cells were observed by microscope. The mRNA levels of LHX4 and DIS3L were detected by real-time fluorescent quantitative PCR, and the protein expression levels of LHX4 and DIS3L were detected by Western blot. Results: when the concentrations of p, p'-DDE were 0, 0.5, 1.0 and 2.0 μg/ml, Nthy-ori-3-1 cells grew normally. There were 33 differential genes in 2.0 μg/ml group, among which 13 genes were down regulated and 20 genes were up-regulated. Compared with the control group, the protein expression levels of LHX4 and DIS3L in 1.0 and 2.0 μg/ml groups were significantly decreased (P<0.05) , and the relative expression levels of LHX4 and DIS3L protein mRNA in 1.0 μg/ml group were significantly decreased (P<0.05) . Conclusion: p, p'-DDE can affect the protein expression of LHX4 and dis3l in nthy-ori-3-1 cells.
Insights
The thyroid disruptor p,p'-DDE significantly decreases LHX4 and DIS3L protein and mRNA expression in Nthy-ori-3-1 cells. This indicates p,p'-DDE impacts cellular processes regulated by these key genes.
Area of Science:
- Endocrinology
- Molecular Biology
- Environmental Toxicology
Background:
- Thyroid disruptors pose risks to human health.
- Understanding the molecular mechanisms of thyroid disruptors is crucial.
Purpose of the Study:
- To investigate the effects of p,p omino'-DDE on LHX4 and DIS3L gene and protein expression.
- To assess the impact of p,p omino'-DDE on Nthy-ori-3-1 cell lines.
Main Methods:
- Nthy-ori-3-1 cells were treated with varying concentrations of p,p omino'-DDE.
- Real-time fluorescent quantitative PCR was used to measure mRNA levels.
- Western blot analysis was performed to quantify protein expression.
Main Results:
- p,p omino'-DDE exposure led to significant decreases in LHX4 and DIS3L protein expression at concentrations of 1.0 and 2.0 μg/ml.
- mRNA levels of LHX4 and DIS3L were also significantly reduced in the 1.0 μg/ml group.
- Gene expression profiling revealed 33 differentially expressed genes at 2.0 μg/ml p,p omino'-DDE.
Conclusions:
- p,p omino'-DDE exposure negatively affects LHX4 and DIS3L expression in Nthy-ori-3-1 cells.
- The study highlights the potential of p,p omino'-DDE to disrupt cellular functions through modulation of these genes.
Related Concept Videos
01:20Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
01:18Functions of Thyroid Hormones
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
02:23Abnormal Proliferation