Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SARS-CoV-2 nucleocapsid protein intranasal inoculation induces local and systemic T cell responses in mice.

Journal of medical virology·2021

Same author

Complete mitochondrial genome of the spotted alfalfa aphid, <i>Therioaphis trifolii</i> (Hemipera: Aphididae).

Mitochondrial DNA. Part B, Resources·2020

Same author

The complete mitochondrial genome of the mealy plum aphid, <i>Hyalopterus pruni</i> (Hemiptera: Aphididae).

Mitochondrial DNA. Part B, Resources·2020

Same author

LncRNA PART1 promotes cell proliferation and progression in non-small-cell lung cancer cells via sponging miR-17-5p.

Journal of cellular biochemistry·2020

Same author

Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization.

Journal of immunology research·2020

Same author

Maternal Methyl-Donor Micronutrient Supplementation During Pregnancy Promotes Skeletal Muscle Differentiation and Maturity in Newborn and Weaning Pigs.

Frontiers in nutrition·2020

Search research articles

Related Experiment Video

Updated: Dec 9, 2025

An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules

An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules

Published on: November 4, 2022

[Ingredients changes in Rehmanniae Radix processing based by HPLC-ELSD specific chromatogram].

Wen-Ting Zhang¹, Jia He², Zeng-Xi Guo¹

¹Zhenjiang Institute for Food and Drug Control Hangzhou 310052, China NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine) Hangzhou 310052, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica

|September 7, 2020

Summary

This summary is machine-generated.

This study developed a HPLC-ELSD method to analyze Rehmanniae Radix and Rehmanniae Radix Praeparata. Processing significantly alters chemical composition, with monosaccharide and oligosaccharide ratios indicating processing quality.

Keywords:

HPLC-ELSD Rehmanniae Radix Rehmanniae Radix Praeparata fingerprint proportion of ingredients

More Related Videos

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

Related Experiment Videos

Last Updated: Dec 9, 2025

An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules

An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules

Published on: November 4, 2022

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

Area of Science:

Pharmacognosy
Analytical Chemistry
Traditional Chinese Medicine

Background:

Rehmanniae Radix Praeparata is a processed form of Rehmannia Radix with altered therapeutic properties.
Understanding the chemical changes during processing is crucial for quality control.

Purpose of the Study:

To establish a High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection (HPLC-ELSD) method for analyzing Rehmannia Radix and its processed form.
To compare the chemical compositions of raw and processed Rehmannia Radix.
To elucidate the compositional changes during processing.

Main Methods:

Utilized HPLC-ELSD with a Prevail Carbohydrate ES column and gradient elution (acetonitrile-water).
Analyzed 23 batches of Rehmannia Radix and 25 batches of Rehmanniae Radix Praeparata samples.
Compared chromatograms to identify common peaks and assess compositional similarity.

Main Results:

The developed HPLC-ELSD method demonstrated excellent repeatability, precision, and stability.
Significant compositional differences were observed between Rehmannia Radix and Rehmanniae Radix Praeparata.
Processing involves the transformation of main oligosaccharides (sucrose, stachyose tetrahydrate) into monosaccharides (fructose, glucose) and other oligosaccharides.

Conclusions:

The ratio of monosaccharides to oligosaccharides serves as a reliable quantitative criterion for assessing the processing quality of Rehmanniae Radix Praeparata.
The established method allows for effective differentiation and quality evaluation of processed Rehmannia Radix.