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Published on: December 4, 2017
[Proteomics and peptidomics analysis of Sepiae Endoconcha]
Rui Liu1, Shuang Wei1, Xin-Zhi Wang1
1Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics Nanjing 210023, China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
This study identified key proteins and peptides in marine traditional Chinese medicine (TCM) cuttlebone using proteomics and peptidomics. These findings provide a foundation for further research into marine TCM components.
Area of Science:
- Marine biology
- Biochemistry
- Traditional Chinese Medicine (TCM)
Background:
- Sepiae Endoconcha (cuttlebone) is a component of marine traditional Chinese medicine.
- Understanding its molecular composition is crucial for its application and research.
Purpose of the Study:
- To identify and characterize the proteins and peptides present in Sepiae Endoconcha.
- To establish a theoretical and research basis for marine TCM cuttlebone.
Main Methods:
- Shotgun proteomics and peptidomics analysis.
- Peptide extraction using acidified methanol and enrichment via strong cation exchange (SCX) resin.
- Protein extraction, trypsin digestion, and analysis using nano-LC Q Exactive Orbitrap mass spectrometry.
Main Results:
- Identification of 16 proteins, including hemocyanin, enolase, myosin, actin, and calmodulin.
- Identification of 168 peptides by database search and 328 peptides by de novo sequencing.
- Identified peptides were derived from actin, histone, and tubulin, representing important cuttlebone components.
Conclusions:
- Proteomics and peptidomics successfully identified significant protein and peptide components in Sepiae Endoconcha.
- The identified molecules are integral to cuttlebone's composition.
- This study offers a foundational understanding for future investigations into marine TCM derived from cuttlebone.
