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Determination of theophylline concentrations by AccuLevel
Q C Nguyen1, R M Sly, R L Boeckx
1Department of Allergy and Immunology, Children's Hospital National Medical Center, Washington, DC.
Annals of Allergy
|June 1, 1988
Summary
AccuLevel and EMIT methods show excellent correlation for measuring theophylline levels in blood. AccuLevel is a precise and rapid assay for therapeutic drug monitoring.
Area of Science:
- Clinical Chemistry
- Pharmacokinetics
- Analytical Toxicology
Background:
- Therapeutic drug monitoring (TDM) is crucial for optimizing drug efficacy and minimizing toxicity.
- Theophylline is an important medication requiring precise concentration measurements.
Purpose of the Study:
- To evaluate the AccuLevel assay against the EMIT method for theophylline determination.
- To assess the precision and accuracy of the AccuLevel method in whole blood samples.
Main Methods:
- Paired venous blood samples (n=29) were analyzed using both AccuLevel and EMIT assays.
- Precision of the AccuLevel method was evaluated through replicate analyses (n=20).
Main Results:
- Excellent correlation was observed between AccuLevel and EMIT measurements.
- AccuLevel demonstrated high precision with a coefficient of variation of 3.5%.
Conclusions:
- AccuLevel is an accurate and precise method for determining theophylline concentrations.
- The AccuLevel assay offers a rapid option for clinical laboratory analysis of theophylline.