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Related Experiment Video

Updated: Dec 9, 2025

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats

Published on: August 22, 2020

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats.

César Acevedo-Triana¹, Javier L Rico², Leonardo A Ortega²

¹School of Psychology, Universidad Pedagógica y Tecnológica de Colombia; Department of Neurobiology, University of Alabama at Birmingham.

Journal of Visualized Experiments : Jove

|September 7, 2020

Summary

This summary is machine-generated.

This study introduces an extended fear-conditioning protocol in rats, demonstrating overtraining and fear incubation. The findings reveal long-lasting fear responses, offering insights into emotional memory and PTSD.

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Area of Science:

Neuroscience
Behavioral Psychology
Animal Models

Background:

Emotional memory is often studied using fear-conditioning paradigms in rats.
Standard protocols involve few tone-shock pairings, but extended training is crucial for understanding fear incubation.

Purpose of the Study:

To describe an extended fear-conditioning protocol that induces overtraining and fear incubation in rats.
To compare conditioned freezing responses at short-term and long-term intervals after training.

Main Methods:

An extended fear-conditioning protocol with 25 tone-shock pairings in a single session was used.
Conditioned freezing responses were assessed in both context and cue tests at 48 hours and 6 weeks post-training.

Main Results:

The protocol successfully induced overtraining and subsequent fear incubation.
Fear responses showed a long-lasting effect, increasing over time without further conditioning.

Conclusions:

The described protocol is effective for studying overtraining and fear incubation in rats.
This model provides valuable insights into the neurobiological mechanisms of long-lasting emotional memories and their relation to conditions like PTSD.