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Vaccine Based on Outer Membrane Vesicles Using Hydrogels as Vaccine Delivery System
Yadira Pastor1, Isaiah Ting1, Melibea Berzosa1
1Department of Microbiology, University of Navarra, Pamplona, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|September 7, 2020
Summary
Outer membrane vesicles from Salmonella enterica were released using heat treatment. Embedding these bacterial antigens into hydrogels may enhance intranasal vaccination efficacy by improving mucosal immune system activation.
Area of Science:
- Microbiology
- Immunology
- Biomaterials Science
Background:
- Outer membrane vesicles (OMVs) are promising vaccine candidates.
- Salmonella enterica is a significant human pathogen.
- Hydrogels offer potential for controlled vaccine delivery.
Purpose of the Study:
- To develop a simple method for isolating OMVs from Salmonella enterica.
- To evaluate the use of hydrogels as a delivery system for these OMVs.
- To assess the potential of hydrogel-encapsulated OMVs for intranasal vaccination.
Main Methods:
- Whole Salmonella enterica bacteria were heat-treated in saline solution to release OMVs.
- OMVs containing antigenic components were isolated.
- Antigens were embedded into hydrogels for intranasal administration.
Main Results:
- A straightforward procedure for obtaining OMVs from Salmonella enterica was established.
- Heat treatment effectively released OMVs with relevant antigenic components.
- Hydrogel encapsulation is proposed to improve antigen residence time and mucosal immune activation.
Conclusions:
- The described method provides a simple means to produce OMVs from Salmonella enterica.
- Hydrogel-based delivery systems show potential for enhancing the immunogenicity of intranasal vaccines.
- This approach may lead to improved mucosal immunity against Salmonella infections.
