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Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
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A Removable Artificial Cell Wall for Withstanding Ciprofloxacin.

Hanjiao Hu1, Xingtang Liang2, Shuangshuang Wang2

  • 1School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.

Macromolecular Bioscience
|September 8, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a protective layer for Saccharomyces cerevisiae to enhance probiotic survival in antibiotic-contaminated aquaculture environments. This cell surface engineering strategy improves probiotic viability against harmful antibiotics.

Keywords:
antibiotic resistanceartificial cell wallcell surface engineeringprobioticsself-assembly

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Area of Science:

  • Biotechnology and Aquaculture

Background:

  • Antibiotic pollution in aquaculture poses a significant threat to the viability of probiotics essential for feed quality.
  • Existing probiotics often fail in antibiotic-contaminated environments, necessitating novel protection strategies.

Purpose of the Study:

  • To develop a method for protecting Saccharomyces cerevisiae cells from antibiotic contamination in aquaculture settings.
  • To enhance the survival rate of probiotics in the presence of antibiotics using cell surface engineering.

Main Methods:

  • Constructed a protective layer on Saccharomyces cerevisiae using self-assembled coacervate microdroplets of carboxymethyl chitosan and carboxyl dextran.
  • Utilized cell surface engineering to create a removable, artificial cell wall around the probiotic cells.

Main Results:

  • The engineered protective layer effectively captured ciprofloxacin, reducing direct contact with Saccharomyces cerevisiae cells.
  • The strategy significantly improved the survival rate of Saccharomyces cerevisiae when exposed to ciprofloxacin without compromising cell viability.

Conclusions:

  • A facile and effective strategy for creating a removable, artificial cell wall using biodegradable polysaccharides was demonstrated.
  • This approach offers a promising solution for enhancing probiotic productivity in antibiotic-polluted aquaculture environments.