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A quick flow cytometry protocol to assess Helicobacter pylori viability.
Claudio Alba1, Alicia C Marin2, Adrian G McNicholl2
1Microbiology Unit, Hospital Universitario de La Princesa and Instituto de Investigación Sanitaria Princesa (IP), Madrid, Spain.
Journal of Microbiological Methods
|September 8, 2020
Summary
We developed a simple flow cytometry method to assess Helicobacter pylori viability, even at low bacterial densities. This technique aids in rapidly evaluating eradication strategies against H. pylori.
Area of Science:
- Microbiology
- Cell Biology
- Immunology
Background:
- Helicobacter pylori is a significant human pathogen.
- Assessing bacterial viability is crucial for understanding infection dynamics and treatment efficacy.
- Current methods for H. pylori viability assessment can be time-consuming or lack sensitivity.
Purpose of the Study:
- To present an accessible flow cytometry protocol for determining Helicobacter pylori viability.
- To enable the detection of low live bacterial densities.
- To provide a tool for rapid assessment of eradication methods.
Main Methods:
- Development of a flow cytometry protocol.
- Staining of Helicobacter pylori with viability dyes.
- Analysis of bacterial populations based on fluorescence intensity.
Main Results:
- The protocol allows for easy and accurate measurement of H. pylori viability.
- Low densities of live H. pylori were successfully detected.
- The method demonstrated potential for assessing eradication efficacy.
Conclusions:
- This flow cytometry protocol offers a straightforward approach to study H. pylori viability.
- The method is sensitive enough to detect low bacterial loads.
- It serves as a valuable tool for evaluating anti-H. pylori therapies.
