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The yeast cell-type-specific repressor alpha 2 acts cooperatively with a non-cell-type-specific protein
C A Keleher1, C Goutte, A D Johnson
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|June 17, 1988
Summary
A novel protein, GRM, binds cooperatively with yeast alpha 2 repressor. This interaction, crucial for gene repression, involves GRM binding the operator center and alpha 2 dimer binding its ends.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Transcriptional Regulation
Background:
- The yeast alpha 2 protein functions as a cell-type-specific transcriptional repressor.
- Alpha 2 protein exerts its function by binding to specific operator sequences upstream of target genes.
Purpose of the Study:
- To identify and characterize a novel protein that interacts with the yeast alpha 2 protein.
- To elucidate the mechanism of cooperative binding and its role in transcriptional repression.
Main Methods:
- Protein-protein interaction studies using yeast genetics.
- Analysis of mutant operators to determine DNA recognition sequences.
- In vivo repression assays using a test promoter.
Main Results:
- A novel protein, designated GRM, was identified that binds cooperatively with alpha 2.
- GRM binds the operator's center, while an alpha 2 dimer binds the operator's ends, creating a unique protein arrangement.
- Both GRM and alpha 2 recognition sequences are essential for repression in vivo.
Conclusions:
- GRM and alpha 2 exhibit cooperative binding at the operator, mediated by protein-protein interactions.
- The N-terminal domain of alpha 2 is involved in the cooperative binding with GRM.
- This interaction is critical for the transcriptional repression mediated by the GRM/alpha 2 complex.
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