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Related Experiment Video

Updated: Dec 9, 2025

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

An Iodinated DAPI-Based Reagent for Mass Cytometry.

Rahul Rana¹, Qing Chang², Jay Bassan^1,3

¹Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.

Chembiochem : a European Journal of Chemical Biology

|September 8, 2020

Summary

This summary is machine-generated.

Researchers developed I-DAPI, a novel heavy-atom probe for mass cytometry (MC) and imaging mass cytometry (IMC™). This new reagent effectively detects cellular DNA, offering a new strategy for developing functional MC and IMC™ reagents.

Keywords:

DNA dye fluorescent probes intercalator iodine mass cytometry

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Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Multiparametric single-cell analysis is advancing with mass cytometry (MC) and imaging mass cytometry (IMC™).
These techniques utilize heavy-isotope-tagged antibodies for simultaneous biomarker identification.
Small-molecule probes with heavy isotopes are increasingly valuable for cellular analysis.

Purpose of the Study:

To explore the iodination of DAPI, a common fluorescent DNA stain, to create a heavy-atom-substituted derivative.
To assess the utility of this novel I-DAPI probe in mass cytometry and imaging mass cytometry assays.
To investigate a strategy for repurposing existing stains into functional MC and IMC™ reagents.

Main Methods:

Chemical synthesis of I-DAPI through iodination of DAPI.
Evaluation of I-DAPI's DNA binding affinity.
Application of I-DAPI in mass cytometry (MC) and imaging mass cytometry (IMC™) assays for cellular DNA detection.

Main Results:

I-DAPI was successfully synthesized as a heavy-atom-substituted DNA stain.
Despite reduced fluorescence, I-DAPI maintained strong DNA binding affinity.
I-DAPI demonstrated comparable efficiency to existing iridium-containing DNA intercalators in MC and IMC™ assays.

Conclusions:

Repurposing well-known colorimetric stains via simple reactions is a viable approach for developing new MC and IMC™ reagents.
I-DAPI serves as a functional heavy-atom probe for cellular DNA detection in advanced cytometry techniques.
This study expands the toolkit for multiparametric single-cell analysis using mass cytometry.