Cisplatin-loaded albumin nanoparticle and study their internalization effect by using β-cyclodextrin.

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Drug Delivery

Background:

• Cisplatin (CPT) is a widely used chemotherapeutic agent, but its efficacy is limited by toxicity and poor targeting.
• Developing effective drug delivery systems is crucial for enhancing the therapeutic properties of anticancer drugs like cisplatin.

Purpose of the Study:

• To enhance the therapeutic and anti-cancer properties of cisplatin by encapsulating it within bovine serum albumin (BSA) nanoparticles (CPT-BSANPs).
• To evaluate the physicochemical characteristics, in vitro drug release, and anti-cancer activity of the developed CPT-BSANPs.

Main Methods:

• CPT-BSANPs were prepared using the desolvation technique.
• Physicochemical characterization was performed using FTIR, SEM, and AFM.
• In vitro drug release and cytotoxicity assays on MCF-7 cell lines were conducted.

Main Results:

• CPT-BSANPs exhibited a particle size below 200 nm with high entrapment efficiency (75.02%).
• In vitro studies showed sustained release of CPT over 48 hours (64% release).
• CPT-BSANPs demonstrated enhanced cytotoxicity and specific targeting towards MCF-7 cells compared to free CPT.

Conclusions:

• CPT-BSANPs are a promising nanocarrier for improving cisplatin's anti-cancer activity.
• The enhanced efficacy suggests that CPT-BSANPs are internalized via caveolae-mediated endocytosis, facilitating intracellular drug delivery.