Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

56.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
56.3K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.3K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlled Synthesis of Chiral β-Chloramines and Aziridines via an Organo- and Biocatalytic Cascade.

Journal of the American Chemical Society·2026
Same author

Development and validation of hypermethylated gene markers in cervical cytological samples for detecting endometrial cancer (EndoMethy-I trial).

Frontiers in oncology·2026
Same author

Correction to:NMI Promotes Pro-Inflammatory Macrophage Polarization and Exacerbates Ulcerative Colitis.

Inflammation·2026
Same author

Molecular signatures and lineage diversification of neurogenic and gliogenic radial glia in the gyrencephalic ferret cortex.

Communications biology·2026
Same author

A Multi-omics Investigation Identifies TACC3 as a Driver of Immunosuppression in Intrahepatic Cholangiocarcinoma via Activation of the STAT3-PD-L1 Axis.

Journal of clinical and translational hepatology·2026
Same author

Heterotrimeric G Protein-RasGAP Coupling Drives Adaptation During Chemotaxis.

Cells·2026

Related Experiment Video

Updated: Dec 9, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.9K

A CRISPR-based method for testing the essentiality of a gene.

Yan You1, Sharmila G Ramachandra1, Tian Jin2

  • 1Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.

Scientific Reports
|September 9, 2020
PubMed
Summary

We developed a CRISPR-based gene essentiality test (CRISPR-E test) to identify essential genes by analyzing mutation types in surviving cells. This method distinguishes essential from non-essential genes based on frameshift mutation survival.

More Related Videos

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.3K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.4K

Related Experiment Videos

Last Updated: Dec 9, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.9K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.3K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.4K

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • The CRISPR/Cas9 system enables precise gene editing by introducing targeted errors.
  • Identifying essential genes is crucial for understanding cellular functions and disease mechanisms.

Purpose of the Study:

  • To introduce a novel CRISPR-based test for determining gene essentiality (CRISPR-E test).
  • To differentiate between essential and non-essential genes based on mutation outcomes.

Main Methods:

  • Utilizing sgRNA-mediated CRISPR/Cas9 to target gene open reading frames.
  • Analyzing in-frame (3n) and frameshift (3n+1, 3n+2) mutations in surviving cells.
  • Validating the CRISPR-E test in Dictyostelium discoideum.

Main Results:

  • Non-essential genes allow both in-frame and frameshift mutations in surviving cells.
  • Essential genes permit only in-frame mutations, as frameshift mutations are eliminated.
  • Demonstrated Dync1li1 as essential, while KIF1A and fAR1 were identified as non-essential in Dictyostelium discoideum.

Conclusions:

  • The CRISPR-E test effectively identifies essential genes by assessing mutation tolerance.
  • This method provides a quantitative approach to measure gene essentiality.
  • The CRISPR-E test offers a powerful tool for genetic research and functional genomics.